Catalog Number - 70000-SP50

The 70000‑SP50 Diamond Hydride Semi‑Preparative HPLC Column is designed for method scale‑up and sample purification when analytical‑scale columns no longer provide sufficient loading capacity. This column enables efficient isolation and collection of milligram‑level quantities of polar compounds while preserving the selectivity and retention characteristics of Diamond Hydride HILIC chemistry.

Semi‑Preparative Use Case: Why 10 mm ID × 50 mm

The 10 mm internal diameter classifies this column as semi‑preparative, making it ideal for laboratories transitioning from analytical HPLC to purification workflows. Compared to 4.6 mm analytical columns, this format:

• Supports significantly higher sample loading

• Enables collection of larger fractions

• Preserves chromatographic behavior during method scale‑up

• Bridges analytical development and full preparative purification

The 50 mm column length emphasizes throughput and efficiency, allowing rapid separations while maintaining sufficient resolution for targeted isolations.

Diamond Hydride™ TYPE‑C Stationary Phase Advantages

Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase provides distinct advantages for semi‑prep HILIC separations:

• Stable, reproducible retention for polar analytes

• Faster equilibration than conventional bonded‑silica HILIC phases

• Robust column lifetime, even under high‑organic mobile‑phase conditions

This chemistry is well suited for purification of:

• Peptides and peptide‑like compounds

• Metabolites and highly polar small molecules

• Analytes containing amine and basic functional groups

• Other compounds requiring HILIC‑based isolation

Made‑to‑Order Columns & Pricing Information

These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:

• Pricing and technical support is provided through our Customer Service Department via formal quotation. Use our online form to start the process or call customer service.

• Columns are produced on order, based on customer requirements

• Orders cannot be returned or canceled once placed

System Requirements

• Typical operating flow rates: ~3–8 mL/min

• Requires a semi‑preparative HPLC system capable of:

  • Higher flow delivery

  • Increased injection volumes

  • Appropriate detector and fraction‑collection support

Standard analytical HPLC systems are not suitable for 10 mm ID semi‑prep columns.

Flow rates may be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.